C++ Mapping::set_rank方法代码示例

void Aligner::align_internal(Alignment& alignment, vector<Alignment>* multi_alignments, Graph& g,
                             int64_t pinned_node_id, bool pin_left, int32_t max_alt_alns, bool print_score_matrices) {

    // check input integrity
    if (pin_left && !pinned_node_id) {
        cerr << "error:[Aligner] cannot choose pinned end in non-pinned alignment" << endl;
        exit(EXIT_FAILURE);
    }
    if (multi_alignments && !pinned_node_id) {
        cerr << "error:[Aligner] multiple traceback is not valid in local alignment, only pinned and global" << endl;
        exit(EXIT_FAILURE);
    }
    if (!(multi_alignments) && max_alt_alns != 1) {
        cerr << "error:[Aligner] cannot specify maximum number of alignments in single alignment" << endl;
        exit(EXIT_FAILURE);
    }


    // alignment pinning algorithm is based on pinning in bottom right corner, if pinning in top
    // left we need to reverse all the sequences first and translate the alignment back later

    // create reversed objects if necessary
    Graph reversed_graph;
    string reversed_sequence;
    if (pin_left) {
        reversed_sequence.resize(alignment.sequence().length());

        reverse_copy(alignment.sequence().begin(), alignment.sequence().end(), reversed_sequence.begin());
        reverse_graph(g, reversed_graph);
    }

    // choose forward or reversed objects
    Graph* align_graph;
    string* align_sequence;
    if (pin_left) {
        align_graph = &reversed_graph;
        align_sequence = &reversed_sequence;
    }
    else {
        align_graph = &g;
        align_sequence = alignment.mutable_sequence();
    }

    // convert into gssw graph and get the counterpart to pinned node (if pinning)
    gssw_node* pinned_node = nullptr;
    gssw_graph* graph = create_gssw_graph(*align_graph, pinned_node_id, &pinned_node);

    if (pinned_node_id & !pinned_node) {
        cerr << "error:[Aligner] pinned node for pinned alignment is not in graph" << endl;
        exit(EXIT_FAILURE);
    }

    // perform dynamic programming
    gssw_graph_fill(graph, (*align_sequence).c_str(),
                    nt_table, score_matrix,
                    gap_open, gap_extension, 15, 2);

    // traceback either from pinned position or optimal local alignment
    if (pinned_node) {
        // trace back pinned alignment
        gssw_graph_mapping** gms = gssw_graph_trace_back_pinned_multi (graph,
                                   pinned_node,
                                   max_alt_alns,
                                   (*align_sequence).c_str(),
                                   (*align_sequence).size(),
                                   nt_table,
                                   score_matrix,
                                   gap_open,
                                   gap_extension);

        if (pin_left) {
            // translate graph and mappings into original node space
            unreverse_graph(reversed_graph);
            for (int32_t i = 0; i < max_alt_alns; i++) {
                unreverse_graph_mapping(gms[i]);
            }
        }

        // convert optimal alignment and store it in the input Alignment object (in the multi alignment,
        // this will have been set to the first in the vector)
        if (gms[0]->score > 0) {
            // have a mapping, can just convert normally
            gssw_mapping_to_alignment(graph, gms[0], alignment, print_score_matrices);
        }
        else {
            // gssw will not identify mappings with 0 score, infer location based on pinning

            Mapping* mapping = alignment.mutable_path()->add_mapping();
            mapping->set_rank(1);

            // locate at the end of the node
            Position* position = mapping->mutable_position();
            position->set_node_id(pinned_node_id);
            position->set_offset(pin_left ? 0 : pinned_node->len);

            // soft clip
            Edit* edit = mapping->add_edit();
            edit->set_to_length(alignment.sequence().length());
            edit->set_sequence(alignment.sequence());
        }
//.........这里部分代码省略.........

void Aligner::gssw_mapping_to_alignment(gssw_graph* graph,
                                        gssw_graph_mapping* gm,
                                        Alignment& alignment,
                                        bool print_score_matrices) {
    alignment.clear_path();
    alignment.set_score(gm->score);
    alignment.set_query_position(0);
    Path* path = alignment.mutable_path();
    //alignment.set_cigar(graph_cigar(gm));

    gssw_graph_cigar* gc = &gm->cigar;
    gssw_node_cigar* nc = gc->elements;
    int to_pos = 0;
    int from_pos = gm->position;
    //cerr << "gm->position " << gm->position << endl;
    string& to_seq = *alignment.mutable_sequence();
    //cerr << "-------------" << endl;

    if (print_score_matrices) {
        gssw_graph_print_score_matrices(graph, to_seq.c_str(), to_seq.size(), stderr);
        //cerr << alignment.DebugString() << endl;
    }

    for (int i = 0; i < gc->length; ++i, ++nc) {

        if (i > 0) from_pos = 0; // reset for each node after the first
        // check that the current alignment has a non-zero length
        gssw_cigar* c = nc->cigar;
        int l = c->length;
        if (l == 0) continue;
        gssw_cigar_element* e = c->elements;

        Node* from_node = (Node*) nc->node->data;
        string& from_seq = *from_node->mutable_sequence();
        Mapping* mapping = path->add_mapping();
        mapping->mutable_position()->set_node_id(nc->node->id);
        mapping->mutable_position()->set_offset(from_pos);
        mapping->set_rank(path->mapping_size());

        //cerr << from_node->id() << ":" << endl;

        for (int j=0; j < l; ++j, ++e) {
            Edit* edit;
            int32_t length = e->length;
            //cerr << e->length << e->type << endl;

            switch (e->type) {
            case 'M':
            case 'X':
            case 'N': {
                // do the sequences match?
                // emit a stream of "SNPs" and matches
                int h = from_pos;
                int last_start = from_pos;
                int k = to_pos;
                for ( ; h < from_pos + length; ++h, ++k) {
                    //cerr << h << ":" << k << " " << from_seq[h] << " " << to_seq[k] << endl;
                    if (from_seq[h] != to_seq[k]) {
                        // emit the last "match" region
                        if (h-last_start > 0) {
                            edit = mapping->add_edit();
                            edit->set_from_length(h-last_start);
                            edit->set_to_length(h-last_start);
                        }
                        // set up the SNP
                        edit = mapping->add_edit();
                        edit->set_from_length(1);
                        edit->set_to_length(1);
                        edit->set_sequence(to_seq.substr(k,1));
                        last_start = h+1;
                    }
                }
                // handles the match at the end or the case of no SNP
                if (h-last_start > 0) {
                    edit = mapping->add_edit();
                    edit->set_from_length(h-last_start);
                    edit->set_to_length(h-last_start);
                }
                to_pos += length;
                from_pos += length;
            }
            break;
            case 'D':
                edit = mapping->add_edit();
                edit->set_from_length(length);
                edit->set_to_length(0);
                from_pos += length;
                break;
            case 'I':
                edit = mapping->add_edit();
                edit->set_from_length(0);
                edit->set_to_length(length);
                edit->set_sequence(to_seq.substr(to_pos, length));
                to_pos += length;
                break;
            case 'S':
                // note that soft clips and insertions are semantically equivalent
                // and can only be differentiated by their position in the read
                // with soft clips coming at the start or end
                edit = mapping->add_edit();
//.........这里部分代码省略.........