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C++ Mapping::is_reverse方法代码示例

本文整理汇总了C++中Mapping::is_reverse方法的典型用法代码示例。如果您正苦于以下问题:C++ Mapping::is_reverse方法的具体用法?C++ Mapping::is_reverse怎么用?C++ Mapping::is_reverse使用的例子?那么, 这里精选的方法代码示例或许可以为您提供帮助。您也可以进一步了解该方法所在Mapping的用法示例。


在下文中一共展示了Mapping::is_reverse方法的1个代码示例,这些例子默认根据受欢迎程度排序。您可以为喜欢或者感觉有用的代码点赞,您的评价将有助于系统推荐出更棒的C++代码示例。

示例1: compute_from_edit

void Pileups::compute_from_edit(NodePileup& pileup, int64_t& node_offset,
                                int64_t& read_offset,
                                const Node& node, const Alignment& alignment,
                                const Mapping& mapping, const Edit& edit) {
    string seq = edit.sequence();
    bool is_reverse = mapping.is_reverse();
    
    // ***** MATCH *****
    if (edit.from_length() == edit.to_length()) {
        assert (edit.from_length() > 0);
        make_match(seq, edit.from_length(), is_reverse);
        assert(seq.length() == edit.from_length());            
        int64_t delta = is_reverse ? -1 : 1;
        for (int64_t i = 0; i < edit.from_length(); ++i) {
            BasePileup* base_pileup = get_create_base_pileup(pileup, node_offset);
            // reference_base if empty
            if (base_pileup->num_bases() == 0) {
                base_pileup->set_ref_base(node.sequence()[node_offset]);
            } else {
                assert(base_pileup->ref_base() == node.sequence()[node_offset]);
            }
            // add base to bases field (converting to ,. if match)
            char base = seq[i];
            if (!edit.sequence().empty() &&
                base_equal(seq[i], node.sequence()[node_offset], is_reverse)) {
                base = is_reverse ? ',' : '.';
            }
            *base_pileup->mutable_bases() += base;
            // add quality if there
            if (!alignment.quality().empty()) {
                *base_pileup->mutable_qualities() += alignment.quality()[read_offset];
            }
            // pileup size increases by 1
            base_pileup->set_num_bases(base_pileup->num_bases() + 1);
            // move right along read, and left/right depending on strand on reference
            node_offset += delta;
            ++read_offset;
        }
    }
    // ***** INSERT *****
    else if (edit.from_length() < edit.to_length()) {
        make_insert(seq, is_reverse);
        assert(edit.from_length() == 0);
        // we define insert (like sam) as insertion between current and next
        // position (on forward node coordinates). this means an insertion before
        // offset 0 is invalid! 
        int64_t insert_offset =  is_reverse ? node_offset : node_offset - 1;
        if (insert_offset >= 0) {        
            BasePileup* base_pileup = get_create_base_pileup(pileup, insert_offset);
            // reference_base if empty
            if (base_pileup->num_bases() == 0) {
                base_pileup->set_ref_base(node.sequence()[insert_offset]);
            } else {
                assert(base_pileup->ref_base() == node.sequence()[insert_offset]);
            }
            // add insertion string to bases field
            // todo: should we reverse complement this if mapping is reversed ??? 
            base_pileup->mutable_bases()->append(seq);
            if (!alignment.quality().empty()) {
                *base_pileup->mutable_qualities() += alignment.quality()[read_offset];
            }
            // pileup size increases by 1
            base_pileup->set_num_bases(base_pileup->num_bases() + 1);
        }
        else {
            // todo: need to either forget about these, or extend pileup format.
            // easy solution: change insert to come before position, and just add
            // optional pileup at n+1st base of node.  would like to figure out
            // how samtools does it first...
            /*
            stringstream ss;
            ss << "Warning: pileup does not support insertions before 0th base in node."
               << " Offending edit: " << pb2json(edit) << endl;
#pragma omp critical(cerr)
            cerr << ss.str();
            */
        }
        // move right along read (and stay put on reference)
        read_offset += edit.to_length();
    }
    // ***** DELETE *****
    else {
        assert(edit.to_length() == 0);
        assert(edit.sequence().empty());
        int64_t del_start = !is_reverse ? node_offset :
            node_offset - edit.from_length() + 1;
        seq = node.sequence().substr(del_start, edit.from_length());
        make_delete(seq, is_reverse);
        BasePileup* base_pileup = get_create_base_pileup(pileup, node_offset);
        // reference_base if empty
        if (base_pileup->num_bases() == 0) {
            base_pileup->set_ref_base(node.sequence()[node_offset]);
        } else {
            assert(base_pileup->ref_base() == node.sequence()[node_offset]);
        }
        // add deletion string to bases field
        // todo: should we reverse complement this if mapping is reversed ??? 
        base_pileup->mutable_bases()->append(seq);
        if (!alignment.quality().empty()) {
            *base_pileup->mutable_qualities() += alignment.quality()[read_offset];
//.........这里部分代码省略.........
开发者ID:gitter-badger,项目名称:vg,代码行数:101,代码来源:pileup.cpp


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