Python ConfigUtils.createConfigParser方法代码示例

# 需要导入模块: from oncotator.utils.ConfigUtils import ConfigUtils [as 别名]
# 或者: from oncotator.utils.ConfigUtils.ConfigUtils import createConfigParser [as 别名]
 def __init__(self, filename, configFile="tcgaVCF1.1_output.config", otherOptions=None):
     self._filename = filename
     self.logger = logging.getLogger(__name__)
     self.config = ConfigUtils.createConfigParser(configFile)
     self.alternativeDictionary = ConfigUtils.buildAlternateKeyDictionaryFromConfig(self.config)
     self.seenDbSNPs = dict()
     self.fieldMap = {}

# 需要导入模块: from oncotator.utils.ConfigUtils import ConfigUtils [as 别名]
# 或者: from oncotator.utils.ConfigUtils.ConfigUtils import createConfigParser [as 别名]
    def __init__(self, filename, configFile="tcgaMAF2.4_output.config", other_options=None):
        """
        """
        options = dict() if other_options is None else other_options

        self._filename = filename
        self.logger = logging.getLogger(__name__)
        self.config = ConfigUtils.createConfigParser(configFile)

        self.logger.info("Building alternative keys dictionary...")
        self.alternativeDictionary = ConfigUtils.buildAlternateKeyDictionaryFromConfig(self.config)

        self.options = options

        self._prepend = self.config.get("general", "prepend")
        if self.options.get(OptionConstants.NO_PREPEND, False):
            self._prepend = ""

        # _is_reannotating is a flag to determine whether we should give precendence to annotations that were not
        #   annotated as part of the INPUT.
        self._is_reannotating = options.get(OptionConstants.REANNOTATE_TCGA_MAF_COLS, False)

        self._is_splitting_allelic_depth = self.options.get(OptionConstants.SPLIT_ALLELIC_DEPTH, True)

        self.exposedColumns = set(self.config.get("general", "exposedColumns").split(','))

        self._is_entrez_id_message_logged = False

        self._is_collapsing_number_cols = options.get(OptionConstants.COLLAPSE_NUMBER_ANNOTATIONS, False)
        self._column_collapser = None
        self._column_collapser_suffix = None
        if self._is_collapsing_number_cols:
            self._column_collapser = ColumnCollapser()
            self._column_collapser_suffix = "_full"

# 需要导入模块: from oncotator.utils.ConfigUtils import ConfigUtils [as 别名]
# 或者: from oncotator.utils.ConfigUtils.ConfigUtils import createConfigParser [as 别名]
    def testCreateDatasourceWithMissingValues(self):
        """

        """
        dsFile = os.path.join("testdata", "ESP6500SI-V2.chr1.snps_indels.head.25.missing.txt")
        destDir = "out"
        datasourceFilename = "ESP6500SI-V2.chr1.snps_indels.head.25.missing.tabix_indexed.txt.gz"
        indexColumnNames = "CHROM,POS,POS"
        dataSourceType = "indexed_tsv"
        dataSourceName = "ESP"
        dataSourceVersion = "6500SI-V2"
        dataSourceMatchMode = "overlap"
        annotationColumnNames = "EA_GTC,DP"
        configFilename = os.path.join("out", "esp_coverage.missing.config")

        datasourceBuilder = TabixIndexedTsvDatasourceCreator()
        datasourceBuilder.createDatasource(destDir, dsFile, indexColumnNames, configFilename, dataSourceType, dataSourceName,
                                           dataSourceVersion, dataSourceMatchMode, annotationColumnNames,
                                           DatasourceInstallUtils.getIndexCols(dataSourceType, indexColumnNames))

        configParser = ConfigUtils.createConfigParser(configFilename)
        self.assertEqual(configParser.get("general", "src_file"), datasourceFilename,
                         "Expected data source src_file is %s but was %s."
                         % (datasourceFilename, configParser.get("general", "src_file")))

        self.assertEqual(configParser.get("data_types", "EA_GTC"), "Float",
                         "Expected EA_GTC data type is %s but was %s."
                         % ("Float", configParser.get("data_types", "EA_GTC")))
        self.assertEqual(configParser.get("data_types", "DP"), "Integer",
                         "Expected DP data type is %s but was %s."
                         % ("Integer", configParser.get("data_types", "DP")))

# 需要导入模块: from oncotator.utils.ConfigUtils import ConfigUtils [as 别名]
# 或者: from oncotator.utils.ConfigUtils.ConfigUtils import createConfigParser [as 别名]
    def test_intitialize(self):
        """Test a simple initialization of an ensembl datasource """
        base_config_location = "testdata/ensembl/saccer/"
        config_parser = ConfigUtils.createConfigParser(base_config_location + "ensembl.config")
        title = config_parser.get("general", "title")
        version = config_parser.get("general", "version")
        src_file = config_parser.get("general", "src_file")

        ensembl_ds = EnsemblTranscriptDatasource(title=title, version=version, src_file=src_file)
        self.assertIsNotNone(ensembl_ds)
        ensembl_ds.set_tx_mode(TranscriptProvider.TX_MODE_BEST_EFFECT)
        self.assertTrue(TranscriptProvider.TX_MODE_BEST_EFFECT == ensembl_ds.get_tx_mode())

# 需要导入模块: from oncotator.utils.ConfigUtils import ConfigUtils [as 别名]
# 或者: from oncotator.utils.ConfigUtils.ConfigUtils import createConfigParser [as 别名]
    def testCreateGPTsvDatasource(self):
        """


        """
        datasourceFilename = "testdata/small_genome_position_tsv_ds/oreganno_trim.hg19.txt"
        datasourceType = "gp_tsv"
        datasourceName = "ORegAnno"
        datasourceFoldername = "ORegAnno"
        datasourceVersion = "UCSC Track"
        genomeBuild = "hg19"
        genomicPositionColumnNames = "hg19.oreganno.chrom,hg19.oreganno.chromStart,hg19.oreganno.chromEnd"

        tmpDir = tempfile.mkdtemp()
        destDir = os.path.join(*[tmpDir, datasourceFoldername, genomeBuild])
        os.makedirs(destDir)

        DatasourceInstallUtils.create_datasource(destDir, datasourceFilename, datasourceFoldername, datasourceName,
                                                 datasourceType, datasourceVersion, genomicPositionColumnNames)

        datasourceFilename = "oreganno_trim.hg19.txt"
        configFilename = os.path.join(*[destDir, "ORegAnno.config"])
        configParser = ConfigUtils.createConfigParser(configFilename)
        self.assertTrue(configParser.has_section("general"), "general section is missing.")
        self.assertTrue(configParser.has_option("general", "type"), "type option is missing in general section.")
        self.assertTrue(configParser.has_option("general", "src_file"),
                        "src_file option is missing in general section.")
        self.assertTrue(configParser.has_option("general", "title"), "title option is missing in general section.")
        self.assertTrue(configParser.has_option("general", "version"), "version option is missing in general section.")
        self.assertTrue(configParser.has_option("general", "genomic_position_cols"),
                        "genomic_position_cols option is missing in general section.")

        self.assertEqual(configParser.get("general", "type"), datasourceType,
                         "Expected data source type is %s but was %s."
                         % (datasourceType, configParser.get("general", "type")))
        self.assertEqual(configParser.get("general", "src_file"), datasourceFilename,
                         "Expected data source src_file is %s but was %s."
                         % (datasourceFilename, configParser.get("general", "src_file")))
        self.assertEqual(configParser.get("general", "title"), datasourceName,
                         "Expected data source title is %s but was %s."
                         % (datasourceName, configParser.get("general", "title")))
        self.assertEqual(configParser.get("general", "version"), datasourceVersion,
                         "Expected data source version is %s but was %s."
                         % (datasourceVersion, configParser.get("general", "version")))
        self.assertEqual(configParser.get("general", "genomic_position_cols"), genomicPositionColumnNames,
                         "Expected data source genomic_position_cols is %s but was %s."
                         % (genomicPositionColumnNames, configParser.get("general", "genomic_position_cols")))

        self.assertTrue(os.path.exists(os.path.join(*[tmpDir, datasourceFoldername, genomeBuild + ".md5"])),
                        "No md5 file was generated.")

        MutUtils.removeDir(tmpDir)

# 需要导入模块: from oncotator.utils.ConfigUtils import ConfigUtils [as 别名]
# 或者: from oncotator.utils.ConfigUtils.ConfigUtils import createConfigParser [as 别名]
 def __init__(self, mut, configFile="sample_name_selection.config", section="SAMPLE_NAME"):
     config = ConfigUtils.createConfigParser(configFile)
     self.logger = logging.getLogger(__name__)
     aliases = ConfigUtils.buildAlternateKeyDictionaryFromConfig(config, section)
     self.configFile=configFile
     sampleAnnotation = self._getAnnotationFromAliases(mut, aliases["sample_name"])
     tumorAnnotation = self._getAnnotationFromAliases(mut, aliases["sample_tumor_name"])
     normalAnnotation = self._getAnnotationFromAliases(mut, aliases["sample_normal_name"])
     source_column = self._getSourceColumn(sampleAnnotation,tumorAnnotation,normalAnnotation)
     self._logSampleNameColumnDescription(source_column, sampleAnnotation, tumorAnnotation, normalAnnotation)
     self.sampleNameGrabber = self._getSampleNameGrabber(source_column, sampleAnnotation, tumorAnnotation, normalAnnotation)
     self.outputAnnotationName = self._deriveOutputAnnotationName(sampleAnnotation)
     self.annotationSource = self._deriveAnnotationSource(source_column)

# 需要导入模块: from oncotator.utils.ConfigUtils import ConfigUtils [as 别名]
# 或者: from oncotator.utils.ConfigUtils.ConfigUtils import createConfigParser [as 别名]
    def testCreateIndexedVcfDatasource(self):
        datasourceFilename = "testdata/vcf/example.vcf"
        datasourceFoldername = "1000Genomes"
        datasourceName = "1000Genomes"
        datasourceType = "indexed_vcf"
        datasourceVersion = "V4.1"
        genomeBuild = "hg19"
        tmpDir = tempfile.mkdtemp()
        destDir = os.path.join(*[tmpDir, datasourceFoldername, genomeBuild])
        os.makedirs(destDir)

        DatasourceInstallUtils.create_datasource(destDir, datasourceFilename, datasourceFoldername, datasourceName,
                                                 datasourceType, datasourceVersion)

        datasourceFilename = "example.tabix_indexed.vcf.gz"
        configFilename = os.path.join(*[destDir, "1000Genomes.config"])
        configParser = ConfigUtils.createConfigParser(configFilename)
        self.assertTrue(configParser.has_section("general"), "general section is missing.")
        self.assertTrue(configParser.has_option("general", "type"), "type option is missing in general section.")
        self.assertTrue(configParser.has_option("general", "src_file"),
                        "src_file option is missing in general section.")
        self.assertTrue(configParser.has_option("general", "title"), "title option is missing in general section.")
        self.assertTrue(configParser.has_option("general", "version"), "version option is missing in general section.")

        self.assertEqual(configParser.get("general", "type"), datasourceType,
                         "Expected data source type is %s but was %s."
                         % (datasourceType, configParser.get("general", "type")))
        self.assertEqual(configParser.get("general", "src_file"), datasourceFilename,
                         "Expected data source src_file is %s but was %s."
                         % (datasourceFilename, configParser.get("general", "src_file")))
        self.assertEqual(configParser.get("general", "title"), datasourceName,
                         "Expected data source title is %s but was %s."
                         % (datasourceName, configParser.get("general", "title")))
        self.assertEqual(configParser.get("general", "version"), datasourceVersion,
                         "Expected data source version is %s but was %s."
                         % (datasourceVersion, configParser.get("general", "version")))

        self.assertTrue(os.path.exists(os.path.join(*[tmpDir, datasourceFoldername, genomeBuild + ".md5"])),
                        "No md5 file was generated.")

        # Data source was created correctly
        tabixIndexedFilename = os.path.join(*[destDir, "example.tabix_indexed.vcf.gz"])
        self.assertTrue(os.path.exists(tabixIndexedFilename), "No index file was generated.")

        vcfReader = vcf.Reader(filename=tabixIndexedFilename, compressed=True, strict_whitespace=True)
        vcfRecords = vcfReader.fetch(chrom=20, start=1230237, end=1230237)
        for vcfRecord in vcfRecords:
            self.assertEqual(vcfRecord.INFO["NS"], 3, "Expected %s but got %s." % (3, vcfRecord.INFO["NS"]))
            self.assertEqual(vcfRecord.INFO["DP"], 13, "Expected %s but got %s." % (13, vcfRecord.INFO["DP"]))

        MutUtils.removeDir(tmpDir)

# 需要导入模块: from oncotator.utils.ConfigUtils import ConfigUtils [as 别名]
# 或者: from oncotator.utils.ConfigUtils.ConfigUtils import createConfigParser [as 别名]
    def getConfigTable(self, configFilename, filename=None):
        """



        :param configFilename:
        :param filename:
        :return:
        """
        configParser = ConfigUtils.createConfigParser(configFilename, ignoreCase=False)
        configTable = VcfOutputConfigTable()
        self.createConfigTableKeys(configParser=configParser, configTable=configTable)

        return configTable

# 需要导入模块: from oncotator.utils.ConfigUtils import ConfigUtils [as 别名]
# 或者: from oncotator.utils.ConfigUtils.ConfigUtils import createConfigParser [as 别名]
    def getConfigTable(self, configFilename, filename):
        """


        :return:
        """
        configParser = ConfigUtils.createConfigParser(configFilename, ignoreCase=False)
        configTable = VcfInputConfigTable()
        vcfReader = vcf.Reader(filename=filename, strict_whitespace=True)

        self.createConfigTableKeys(configParser=configParser, configTable=configTable)
        self.createConfigTable(vcfReader=vcfReader, configTable=configTable)

        return configTable

# 需要导入模块: from oncotator.utils.ConfigUtils import ConfigUtils [as 别名]
# 或者: from oncotator.utils.ConfigUtils.ConfigUtils import createConfigParser [as 别名]
    def _validateTcgaMafContents(self, filename):
        """ This is a utility, private method for unit tests to get a semblance that a valid maf file was created.  
        
        Note: This method has nothing to do with the TCGA validator.
        
        """
        configFile = ConfigUtils.createConfigParser(os.path.join("configs", "tcgaMAF2.4_output.config"))
        statinfo = os.stat(filename)
        self.assertTrue(statinfo.st_size > 0, "Generated MAF file (" + filename + ") is empty.")

        tsvReader = GenericTsvReader(filename)

        self.assertTrue(tsvReader.getComments().find('#version') != -1, "First line did not specify a version number")

        ctr = 1
        for lineDict in tsvReader:

            # TODO: Re-enable when GENCODE and HGNC datasources are concordant (or Entrez_Gene_ID is in the gencode gtf)
            # if lineDict['Entrez_Gene_Id'] == "0":
            #     self.assertTrue(lineDict['Hugo_Symbol'] == "Unknown", "Entrez_Gene_Id was zero, but Hugo Symbol was not 'Unknown'.  Line: " + str(ctr))

            unknownKeys = []
            self.assertTrue(lineDict["Tumor_Seq_Allele1"] != lineDict["Tumor_Seq_Allele2"], "Reference and alternate were equal in TCGA MAF output on line %d (%s)" % (ctr, lineDict["Tumor_Seq_Allele1"]))
            self.assertTrue(lineDict["Tumor_Seq_Allele1"] == lineDict["Reference_Allele"], "Reference Allele should match Tumor_Seq_Allele1 on line " + str(ctr))
            uniprot_aa_xform_counter = 0
            for k in lineDict.keys():
                if lineDict[k] == "__UNKNOWN__":
                    unknownKeys.append(k)

                self.assertTrue('\r' not in lineDict[k], "Carriage return character found in an annotation value.")

                requiredColumns = configFile.get("general", "requiredColumns")
                optionalColumns = configFile.get("general", "optionalColumns")
                exposedColumns = configFile.get("general", "exposedColumns")
                if (k not in requiredColumns) and (k not in optionalColumns) and (k not in exposedColumns):
                    self.assertTrue(k.startswith("i_"), "Internal column was not prepended with 'i_'")
            if lineDict['UniProt_AApos'] == "0":
                uniprot_aa_xform_counter += 1

            if lineDict["Variant_Type"] == VariantClassification.VT_DEL:
                self.assertTrue(lineDict["Tumor_Seq_Allele2"] == "-")

            if lineDict["Variant_Type"] == VariantClassification.VT_INS:
                self.assertTrue(lineDict["Reference_Allele"] == "-")

            unknownKeys.sort()
            self.assertTrue(len(unknownKeys) == 0, "__UNKNOWN__ values (" + str(len(unknownKeys)) + ") seen on line " + str(ctr) + ", in fields: " + ", ".join(unknownKeys))
            self.assertTrue(uniprot_aa_xform_counter < 10, "Too many uniprot aa xform values are zero (" + str(uniprot_aa_xform_counter) + ").  This is probably an error.")

            ctr += 1

# 需要导入模块: from oncotator.utils.ConfigUtils import ConfigUtils [as 别名]
# 或者: from oncotator.utils.ConfigUtils.ConfigUtils import createConfigParser [as 别名]
    def __init__(self, filename, mutation_data_factory=None, configFile='maflite_input.config', genomeBuild="hg19", other_options=None):
        """
        Constructor

        """

        super(MafliteInputMutationCreator, self).__init__(filename, mutation_data_factory, configFile, genomeBuild, other_options)

        self.logger = logging.getLogger(__name__)

        self.config = ConfigUtils.createConfigParser(configFile)
        self._tsvReader = GenericTsvReader(filename)
        
        # Key is the required columns and the values are a list of valid alternative headers.
        # Key is column name to an alternative.
        self._alternativeDict = ConfigUtils.buildAlternateKeyDictionaryFromConfig(self.config)
        self._reverseAlternativeDict = ConfigUtils.buildReverseAlternativeDictionary(self._alternativeDict)
        
        missingRequiredHeaders = []
        required_columns = sorted(self.config.get("general", "required_headers").split(","))
        self._build = genomeBuild

        self.logger.info("Initializing a maflite file with the following header: " + str(self._tsvReader.getFieldNames()))

        # The specified fields are those that were given in the input.
        self._specified_fields = self._tsvReader.getFieldNames()

        for col in required_columns:
            if col not in self._specified_fields:
                isAltFound = False
                for alt in self._alternativeDict.get(col, []):
                    if alt in self._specified_fields:
                        isAltFound = True
                        break
                if not isAltFound:

                    # build is optional.
                    if col != "build":
                        missingRequiredHeaders.append(col)
        missingRequiredHeaders.sort()

        if len(missingRequiredHeaders) > 0:
            raise MafliteMissingRequiredHeaderException("Specified maflite file (" + filename + ") missing required headers: " + ",".join(missingRequiredHeaders)  )

# 需要导入模块: from oncotator.utils.ConfigUtils import ConfigUtils [as 别名]
# 或者: from oncotator.utils.ConfigUtils.ConfigUtils import createConfigParser [as 别名]
    def test_simple_annotate(self):
        """ Annotate a simple example.
        """
        base_config_location = "testdata/ensembl/saccer/"
        config_parser = ConfigUtils.createConfigParser(base_config_location + "ensembl.config")
        title = config_parser.get("general", "title")
        version = config_parser.get("general", "version")
        src_file = config_parser.get("general", "src_file")

        ensembl_ds = EnsemblTranscriptDatasource(title=title, version=version, src_file=src_file)

        m = MutationData()
        m.chr = "22"
        m.start = "22161963"
        m.end = "22161963"
        m.ref_allele = "C"
        m.alt_allele = "A"

        m2 = ensembl_ds.annotate_mutation(m)

# 需要导入模块: from oncotator.utils.ConfigUtils import ConfigUtils [as 别名]
# 或者: from oncotator.utils.ConfigUtils.ConfigUtils import createConfigParser [as 别名]
    def _validateTcgaMafContents(self, filename):
        """
        This is a utility, private method for unit tests to get a semblance that a valid maf file was created.
        
        Note: This method has nothing to do with the TCGA validator.
        
        TODO: This is code duplication from TCGA MAF Output RendererTest.  This should be refactored into a base class
        (to preserve self.assertTrue, etc).
        """
        statinfo = os.stat(filename)
        self.assertTrue(statinfo.st_size > 0, "Generated MAF file (" + filename + ") is empty.")

        tsvReader = GenericTsvReader(filename)

        self.assertTrue(tsvReader.getComments().find('#version') <> -1, "First line did not specify a version number")

        ctr = 1
        for lineDict in tsvReader:
            if lineDict['Entrez_Gene_Id'] == "0":
                self.assertTrue(lineDict['Hugo_Symbol'] == "Unknown",
                                "Entrez_Gene_Id was zero, but Hugo Symbol was not 'Unknown'.  Line: " + str(ctr))

            unknownKeys = []
            for k in lineDict.keys():
                if lineDict[k] == "__UNKNOWN__":
                    unknownKeys.append(k)

                self.assertTrue('\r' not in lineDict[k], "Carriage return character found in an annotation value.")

                configFile = ConfigUtils.createConfigParser('configs/tcgaMAF2.3_output.config')
                requiredColumns = configFile.get("general", "requiredColumns")
                optionalColumns = configFile.get("general", "optionalColumns")
                if (k not in requiredColumns) and (k not in optionalColumns):
                    self.assertTrue(k.startswith("i_"), "Internal column was not prepended with 'i_'")

            unknownKeys.sort()
            self.assertTrue(len(unknownKeys) == 0,
                            "__UNKNOWN__ values (" + str(len(unknownKeys)) + ") seen on line " + str(
                                ctr) + ", in fields: " + ", ".join(unknownKeys))

            ctr += 1

# 需要导入模块: from oncotator.utils.ConfigUtils import ConfigUtils [as 别名]
# 或者: from oncotator.utils.ConfigUtils.ConfigUtils import createConfigParser [as 别名]
    def testCreateGPTsvConfigFile(self):
        configFilename = "out/ccle_by_gp.config"
        datasourceFilename = "ccle_results_by_pos.hg19.import.txt"
        dataSourceType = "gp_tsv"
        dataSourceName = "CCLE_By_GP"
        dataSourceVersion = "09292010"
        genomicPositionColumnNames = "chr,start,end"

        datasourceBuilder = GenericTsvDatasourceCreator()
        datasourceBuilder._createConfigFile(configFilename=configFilename, baseDSFile=datasourceFilename,
                                           ds_name=dataSourceName, ds_type=dataSourceType, ds_version=dataSourceVersion,
                                           indexCols=DatasourceInstallUtils.getIndexCols("gp_tsv",
                                                                                         genomicPositionColumnNames))

        configParser = ConfigUtils.createConfigParser(configFilename)
        self.assertTrue(configParser.has_section("general"), "general section is missing.")
        self.assertTrue(configParser.has_option("general", "type"), "type option is missing in general section.")
        self.assertTrue(configParser.has_option("general", "src_file"),
                        "src_file option is missing in general section.")
        self.assertTrue(configParser.has_option("general", "title"), "title option is missing in general section.")
        self.assertTrue(configParser.has_option("general", "version"), "version option is missing in general section.")
        self.assertTrue(configParser.has_option("general", "genomic_position_cols"),
                        "genomic_position_cols option is missing in general section.")

        self.assertEqual(configParser.get("general", "type"), dataSourceType,
                         "Expected data source type is %s but was %s."
                         % (dataSourceType, configParser.get("general", "type")))
        self.assertEqual(configParser.get("general", "src_file"), datasourceFilename,
                         "Expected data source src_file is %s but was %s."
                         % (datasourceFilename, configParser.get("general", "src_file")))
        self.assertEqual(configParser.get("general", "title"), dataSourceName,
                         "Expected data source title is %s but was %s."
                         % (dataSourceName, configParser.get("general", "title")))
        self.assertEqual(configParser.get("general", "version"), dataSourceVersion,
                         "Expected data source version is %s but was %s."
                         % (dataSourceVersion, configParser.get("general", "version")))
        self.assertEqual(configParser.get("general", "genomic_position_cols"), genomicPositionColumnNames,
                         "Expected data source genomic_position_cols is %s but was %s."
                         % (genomicPositionColumnNames, configParser.get("general", "genomic_position_cols")))

# 需要导入模块: from oncotator.utils.ConfigUtils import ConfigUtils [as 别名]
# 或者: from oncotator.utils.ConfigUtils.ConfigUtils import createConfigParser [as 别名]
    def __init__(self, filename, configFile='maflite_input.config', genomeBuild="hg19", other_options=None):
        """
        Constructor

        Currently, this InputCreator does not support any other options.  The parameter is ignored.

        """
        self.logger = logging.getLogger(__name__)

        self.config = ConfigUtils.createConfigParser(configFile)
        self._tsvReader = GenericTsvReader(filename)
        
        # Key is the required columns and the values are a list of valid alternative headers.
        # Key is column name to an alternative.
        self._alternativeDict = ConfigUtils.buildAlternateKeyDictionaryFromConfig(self.config)
        self._reverseAlternativeDict = ConfigUtils.buildReverseAlternativeDictionary(self._alternativeDict)
        
        missingRequiredHeaders = []
        specifiedFields = self._tsvReader.getFieldNames()
        required_columns = sorted(self.config.get("general", "required_headers").split(","))
        self._build = genomeBuild

        for col in required_columns:
            if col not in specifiedFields:
                isAltFound = False
                for alt in self._alternativeDict.get(col, []):
                    if alt in specifiedFields:
                        isAltFound = True
                        break
                if not isAltFound:

                    # build is optional.
                    if col != "build":
                        missingRequiredHeaders.append(col)
        missingRequiredHeaders.sort()
        
        self.logger.info("Initializing a maflite file with the following header: " + str(self._tsvReader.getFieldNames()))
        if len(missingRequiredHeaders) > 0:
            raise MafliteMissingRequiredHeaderException("Specified maflite file (" + filename + ") missing required headers: " + ",".join(missingRequiredHeaders)  )