本文整理汇总了Python中autoprotocol.protocol.Protocol.oligosynthesize方法的典型用法代码示例。如果您正苦于以下问题：Python Protocol.oligosynthesize方法的具体用法？Python Protocol.oligosynthesize怎么用？Python Protocol.oligosynthesize使用的例子？那么恭喜您, 这里精选的方法代码示例或许可以为您提供帮助。您也可以进一步了解该方法所在类autoprotocol.protocol.Protocol的用法示例。
在下文中一共展示了Protocol.oligosynthesize方法的1个代码示例，这些例子默认根据受欢迎程度排序。您可以为喜欢或者感觉有用的代码点赞，您的评价将有助于系统推荐出更棒的Python代码示例。
示例1: get_synthesized_oligo_tube_protocol
# 需要导入模块: from autoprotocol.protocol import Protocol [as 别名]
# 或者: from autoprotocol.protocol.Protocol import oligosynthesize [as 别名]
def get_synthesized_oligo_tube_protocol(tube_name, sequence):
"""
Synthesize and prepare an oligo tube
25nm seems to be more than enough for most downstream work (25pmol seems to be all that is used for transcriptic's pcr)
"""
global inv
scale = '25nm'
p = Protocol()
dna_tube = p.ref(tube_name,
cont_type="micro-1.5",
storage="cold_20", discard=False)
dna_tube.well(0).properties = {'Molar Concentration':'100uM',
'original moles':'25nm'}
p.oligosynthesize([{"sequence": sequence,
"destination": dna_tube.well(0),
"scale": scale,
"purification": "standard"}]
)
#spin
p.spin(dna_tube, '2000:g', '30:second')
#dilute to 100uM
#safe min volume of 1.5uL is 20uL so we want a lot more than this so we don't lose too much
#how do you go from scale and desired concentration to volume --> n = CV --> V = n/C --> V = 25nmol / 100uM = 25nmol / (100E3 nmol / 1L)
# = 2.5E-4 L = 250 uL
#convert 100uM to nM #convert to uL
te_volume = 25 / (100 * pow(10, 3)) * pow(10, 6)
#add 250uL
p.provision(inv["te"], dna_tube.well(0), ul(te_volume))
#spin
p.spin(dna_tube, '2000:g', '30:second')
return json.dumps(p.as_dict(), indent=2)