Python morphology.remove_small_objects函数代码示例

 def get_rough_detection(self, img, bigsize=40.0, smallsize=4.0, thresh = 0):
     diff = self.difference_of_gaussian(-img, bigsize, smallsize)
     diff[diff>thresh] = 1
     
     se = morphology.square(4)
     ero = morphology.erosion(diff, se)
     
     labimage = label(ero)
     #rec = morphology.reconstruction(ero, img, method='dilation').astype(np.dtype('uint8'))
     
     # connectivity=1 corresponds to 4-connectivity.
     morphology.remove_small_objects(labimage, min_size=600, connectivity=1, in_place=True)
     #res = np.zeros(img.shape)
     ero[labimage==0] = 0
     ero = 1 - ero
     labimage = label(ero)
     morphology.remove_small_objects(labimage, min_size=400, connectivity=1, in_place=True)
     ero[labimage==0] = 0
     res = 1 - ero
     res[res>0] = 255
     
     #temp = 255 - temp
     #temp = morphology.remove_small_objects(temp, min_size=400, connectivity=1, in_place=True)
     #res = 255 - temp
     
     return res

def polylinesFromBinImage(img, minimum_cluster_size=6,
                          remove_small_obj_size=3,
                          reconnect_size=3,
                          max_n_contours=None, max_len_contour=None,
                          copy=True):
    '''
    return a list of arrays of un-branching contours

    img -> (boolean) array 

    optional:
    ---------
    minimum_cluster_size -> minimum number of pixels connected together to build a contour

    ##search_kernel_size -> TODO
    ##min_search_kernel_moment -> TODO

    numeric:
    -------------
    max_n_contours -> maximum number of possible contours in img
    max_len_contour -> maximum contour length

    '''
    assert minimum_cluster_size > 1
    assert reconnect_size % 2, 'ksize needs to be odd'

    # assert search_kernel_size == 0 or search_kernel_size > 2 and search_kernel_size%2, 'kernel size needs to be odd'
    # assume array size parameters, is not given:
    if max_n_contours is None:
        max_n_contours = max(img.shape)
    if max_len_contour is None:
        max_len_contour = sum(img.shape[:2])
    # array containing coord. of all contours:
    contours = np.zeros(shape=(max_n_contours, max_len_contour, 2),
                        dtype=np.uint16)  # if not search_kernel_size else np.float32)

    if img.dtype != np.bool:
        img = img.astype(bool)
    elif copy:
        img = img.copy()

    if remove_small_obj_size:
        remove_small_objects(img, remove_small_obj_size,
                             connectivity=2, in_place=True)
    if reconnect_size:
        # remove gaps
        maximum_filter(img, reconnect_size, output=img)
        # reduce contour width to 1
        img = skeletonize(img)

    n_contours = _populateContoursArray(img, contours, minimum_cluster_size)
    contours = contours[:n_contours]

    l = []
    for c in contours:
        ind = np.zeros(shape=len(c), dtype=bool)
        _getValidInd(c, ind)
        # remove all empty spaces:
        l.append(c[ind])
    return l

	def predict(self, times, frames, output_times):
		nout = len(output_times)
		nf = len(frames)

		last_idx = np.argmax(times)
		zt = frames[last_idx] < self.thresh
		skmorph.remove_small_objects(zt, min_size=self.min_size, in_place=True)
		d = morphology.distance_transform_edt(np.invert(zt))
		return np.tile(d, (nout,1,1))

def build_skeleton(frame):
    """
    build a corner tree, skeletonize, dilate
    """
    tree = trees.tree_corners(frame)
    tree = morphology.skeletonize(tree)
    # tree = morphology.binary_dilation(tree)
    morphology.remove_small_objects(tree, min_size=20, connectivity=2, in_place=True)
    tree = morphology.binary_dilation(tree)
    return tree

def distance_trend(times, frames, threshold=25, min_size=12):
	nf = len(frames)
	zt = filters.gaussian_filter(frames,1.5) > threshold
	d_outer = np.zeros((nf,) + frames[0].shape)
	d_inner = np.zeros((nf,) + frames[0].shape)

	for i in range(nf):
		skmorph.remove_small_objects(zt, min_size=min_size, in_place=True)
		d_outer[i] = morphology.distance_transform_edt(np.invert(zt[i]))
		d_inner[i] = morphology.distance_transform_edt(zt[i])

	return d_outer, d_inner

def nuclei_regions(comp_map):
    """
    NUCLEI_REGIONS: extract "support regions" for nuclei. This function
    expects as input a "tissue components map" (as returned, for example,
    by segm.tissue_components) where values of 1 indicate pixels having
    a color corresponding to nuclei.
    It returns a set of compact support regions corresponding to the
    nuclei.


    :param comp_map: numpy.ndarray
       A mask identifying different tissue components, as obtained
       by classification in RGB space. The value 0

       See segm.tissue.tissue_components()

    :return:
    """
    # Deprecated:...
    # img_hem, _ = rgb2he(img0, normalize=True)

    # img_hem = denoise_tv_bregman(img_hem, HE_OPTS['bregm'])

    # Get a mask of nuclei regions by unsupervised clustering:
    # Vector Quantization: background, mid-intensity Hem and high intensity Hem
    # -train the quantizer for 3 levels
    # vq = KMeans(n_clusters=3)
    # vq.fit(img_hem.reshape((-1,1)))
    # -the level of interest is the brightest:
    # k = np.argsort(vq.cluster_centers_.squeeze())[2]
    # mask_hem = (vq.labels_ == k).reshape(img_hem.shape)
    # ...end deprecated

    # Final mask:
    mask = (comp_map == 1)   # use the components classified by color

    # mask = morph.closing(mask, selem=HE_OPTS['strel1'])
    # mask = morph.opening(mask, selem=HE_OPTS['strel1'])
    # morph.remove_small_objects(mask, in_place=True)
    # mask = (mask > 0)

    mask = mahotas.close_holes(mask)
    morph.remove_small_objects(mask, in_place=True)

    dst  = mahotas.stretch(mahotas.distance(mask))
    Bc=np.ones((9,9))
    lmax = mahotas.regmax(dst, Bc=Bc)
    spots, _ = mahotas.label(lmax, Bc=Bc)
    regions = mahotas.cwatershed(lmax.max() - lmax, spots) * mask

    return regions
# end NUCLEI_REGIONS

def stk_to_rois(stk, threshold, min_size, max_window=8, downscale_factor=2):
    thresholded_stk = stk > threshold
    thresholded_stk = remove_small_objects(thresholded_stk, min_size)
    distance = ndi.distance_transform_edt(thresholded_stk)
    cropped_stk = stk.copy()
    cropped_stk[np.logical_not(thresholded_stk)] = 0
    combined_stk = cropped_stk + distance/distance.max()
    local_max = peak_local_max(combined_stk, indices=False, 
                               footprint=np.ones((max_window, max_window)), 
                               labels=thresholded_stk)
    markers = ndi.label(local_max)[0]
    labels = watershed(-combined_stk, markers, mask=thresholded_stk)
    new_markers = markers.copy()
    for i in set(labels.flatten()):
        if i == 0: continue
        if np.sum(labels==i) < min_size:
            new_markers[markers==i] = 0
    labels = watershed(-combined_stk, new_markers, mask=thresholded_stk)
    labels_set = set(labels.flatten())
    rois = []
    for label in labels_set:
        if label == 0: continue
        if np.sum((labels==label).astype(int)) < min_size: continue
        nroi = np.zeros((stk.shape[0], stk.shape[1]))
        cx,cy = np.where(labels==label)
        cx,cy = int(cx.mean()), int(cy.mean())
        x,y = np.ogrid[0:nroi.shape[0], 0:nroi.shape[1]]
        r = 4
        mask =  (cx-x)**2 + (cy-y)**2 <= r*r
        nroi[mask] = 1
        #nroi[labels==label] = 1
        rois.append(zoom(nroi, downscale_factor, order=0))
    rois = np.array(rois)
    return rois, thresholded_stk, labels

def segment_roi(roi):
    # step 1. phase congruency (edge detection)
    Mm = phasecong_Mm(roi)
    # step 2. hysteresis thresholding (of edges)
    B = hysthresh(Mm,HT_T1,HT_T2)
    # step 3. trim pixels off border
    B[B[:,1]==0,0]=0
    B[B[:,-2]==0,-1]=0
    B[0,B[1,:]==0]=0
    B[-1,B[-2,:]==0]=0
    # step 4. threshold to find dark areas
    dark = dark_threshold(roi, DARK_THRESHOLD_ADJUSTMENT)
    # step 5. add dark areas back to blob
    B = B | dark
    # step 6. binary closing
    B = binary_closing(B,SE3)
    # step 7. binary dilation
    B = binary_dilation(B,SE2)
    # step 8. thinning
    B = bwmorph_thin(B,3)
    # step 9. fill holes
    B = binary_fill_holes(B)
    # step 10. remove blobs smaller than BLOB_MIN
    B = remove_small_objects(B,BLOB_MIN,connectivity=2)
    # done.
    return B

def get_bg_mask(img):
    
    #if img.ndim == 3:
    #    bg_mask = img.any(axis=-1)
    #    bg_mask = np.invert(bg_mask) # consistent with np.ma, True if masked

    #    # make multichannel (is it really this hard?)
    #    bg_mask = np.repeat(bg_mask[:,:,np.newaxis], 3, axis=2) 
    #
    #else:
    #    bg_mask = (img != 0)
    #    bg_mask = np.invert(bg_mask) # see above

    #bound = segmentation.find_boundaries(bg_mask, mode='inner', background=1)
    #bg_mask[bound] = 1
    #min_size = img.shape[0] * img.shape[1] // 4 
    #holes = morphology.remove_small_holes(bg_mask, min_size=min_size)
    #bg_mask[holes] = 1
    
    bg_mask = segmentation.find_boundaries(img)
    bg_mask = morphology.remove_small_objects(bg_mask)
    bg_mask = morphology.remove_small_holes(bg_mask)

    bg_mask = np.invert(bg_mask)
    return bg_mask

    def load_cell_image(self, sensitivity = 5., min_cell_size = 4000):
        '''Load cell image and add cells to self'''

        pic_nuclei = self.get_source_pic_nuclei()
        self.shape = pic_nuclei.shape

        nuclei = find_nuclei(pic_nuclei, sensitivity, min_cell_size)

        self.cell_detect_params = (sensitivity, min_cell_size)

        labels = measure_label(nuclei)

        labelcount = np.bincount(labels.ravel())

        bg = np.argmax(labelcount)

        labels += 1

        labels[labels == bg + 1] = 0

        labels = remove_small_objects(labels, min_cell_size)

        self.nuclei = labels

        self.create_cells_from_nuclei(pic_nuclei)

        self.rescale_nuclei()

def pred_f(image, stepSize=stepSize, windowSize=windowSize, param=param, 
           marge=None, marge_cut_off=0, ClearSmallObjects=20, list_f=list_f):
    caffe.set_mode_cpu()
    cn_1 = "FCN_0.01_0.99_0.0005"
    wd_1 = "/share/data40T_v2/Peter/pretrained_models"
    net_1 = GetNet(cn_1, wd_1)
    cn_2 = "DeconvNet_0.01_0.99_0.0005"
    net_2 = GetNet(cn_2, wd_1)
    prob_image, bin_image, thresh = pred_image_from_two_nets(image, net_1, net_2, stepSize, windowSize, 
                                                               param=param, marge=marge, method="avg", 
                                                               ClearBorder="Reconstruction")

    segmentation_mask = DynamicWatershedAlias(prob_image, param)
    segmentation_mask = remove_small_objects(segmentation_mask, ClearSmallObjects)
    table = bin_analyser(image, segmentation_mask, list_f, marge_cut_off)
    segmentation_mask[segmentation_mask > 0] = 1.


    contours = dilation(segmentation_mask, disk(2)) - \
        erosion(segmentation_mask, disk(2))
    x, y = np.where(contours == 1)
    image[x, y] = np.array([0, 0, 0])


    segmentation_mask = img_as_ubyte(segmentation_mask)
    segmentation_mask[segmentation_mask > 0] = 255
    if marge_cut_off != 0:
         c = marge_cut_off
         image = image[c:-c, c:-c]
         segmentation_mask = segmentation_mask[c:-c, c:-c]
         prob_image = prob_image[c:-c, c:-c]
    return image, table, segmentation_mask, prob_image

def cleanImage(img, min_size, scale_factor, img_otsu=None, saver=lambda n,x: x):
    img, exposure_data = normalize_exposure2(img)
    img = saver("01-exposure", img)
    img_otsu = ski.filter.threshold_otsu(img) if not img_otsu else img_otsu
    print('otsu:',img_otsu, ski.filter.threshold_otsu(img))

    img = saver("02-zoom", scipy.ndimage.zoom(img, scale_factor, order=3))
    print("shape after zoom:", img.shape)
#     img_pil = PIL.Image.fromarray(img).resize((np.array(img.shape)*scale_factor).tolist()[:2], resample=PIL.Image.BICUBIC)
#     img = saver("02-zoom", PIL2array(img_pil))
    print("img:",img.shape,img.dtype)

    img_cleaned = saver("03-bw", (img > img_otsu))
#     img_cleaned = saver("03-bw", (img > 0.2))

    dbg = DebugData()

    img_cleaned = morphology.binary_erosion(img_cleaned,morphology.disk(int(2*scale_factor)))
    img_cleaned = saver("04-erosion", img_cleaned, dbg=dbg)

    img_cleaned = morphology.remove_small_objects(img_cleaned, min_size=int(min_size*scale_factor), connectivity=2)
    img_cleaned = saver("05-remove", img_cleaned)


    cleaned_sum = np.sum(img_cleaned)
    print("img_cleaned size:",cleaned_sum)
#     if cleaned_sum < 1000 or cleaned_sum > 300000:
#         display(Image(str(dbg.saved_path)))
#         raise Exception("Image not cleaned correctly"+str(locals()))

    return img_cleaned, exposure_data

def blobs(image, remove_mb = None, val = 160, size = 100):
    """ Convolve a kernel on the image and a gaussian filter to highligh blobs. Find blobs using the
    Difference of Gaussian. Remove from the list of blobs the blobs that are at the membrane.
    return 3 different list
    """

    thresh = threshold_otsu(image)

    #Find all the blobs in the image using Difference of Gaussian
    blobs_in_image = feature.blob_dog(image, min_sigma=0.01,
                        max_sigma=3, threshold=thresh)
    blob_list = []
    for blob in blobs_in_image:
        y, x, r = blob
        blob_list.append((y, x))



    if remove_mb == None:
        blob_in_image_after_binary = set(blob_list)

    else:
        #Create a mask to remove blobs that are at the membrane and surrounded
        #by bright big object
        binary = image >= val*thresh/100
        binary = dilation(binary, square(3))
        binary = remove_small_objects(binary, min_size=size)
        # Create a list of coordinate with the binary image
        coor_binary = np.nonzero(binary)
        list_blob_masked = zip(*coor_binary)
        #Substract the list of coordinate from the binary image to the list of blobs
        blob_in_image_after_binary = (set(blob_list) - set (list_blob_masked))

    return blob_in_image_after_binary

def label_nuclei(binary, min_size):
    '''Label, watershed and remove small objects'''

    distance = medial_axis(binary, return_distance=True)[1]

    distance_blured = gaussian_filter(distance, 5)

    local_maxi = peak_local_max(distance_blured, indices=False, labels=binary, min_distance = 30)

    markers = measure_label(local_maxi)

#    markers[~binary] = -1

#    labels_rw = segmentation.random_walker(binary, markers)

#    labels_rw[labels_rw == -1] = 0

#    labels_rw = segmentation.relabel_sequential(labels_rw)

    labels_ws = watershed(-distance, markers, mask=binary)

    labels_large = remove_small_objects(labels_ws,min_size)

    labels_clean_border = clear_border(labels_large)

    labels_from_one = relabel_sequential(labels_clean_border)

#    plt.imshow(ndimage.morphology.binary_dilation(markers))
#    plt.show()

    return labels_from_one[0]

 def func(frame):
     frame = frame.astype(bool)
     binary = remove_small_objects(frame, smooth_size)
     #binary = ndi.binary_fill_holes(binary)
     #opened = binary_opening(frame, disk(smooth_size))
     #opened = opened & frame
     return binary