Python sequences.file_reader函数代码示例

def run(description):
    parser = argparse.ArgumentParser(
        description = 'Takes a random subset of reads from a sequence file and optionally the corresponding read ' +
                      'from a mates file.  Output is interleaved if mates file given',
        usage = 'fastaq to_random_subset [options] <infile> <outfile> <percent>')
    parser.add_argument('--mate_file', help='Name of mates file')
    parser.add_argument('--seed', help='Seed for random number generator. If not given, python\'s default is used', metavar='INT')
    parser.add_argument('infile', help='Name of input file')
    parser.add_argument('outfile', help='Name of output file')
    parser.add_argument('percent', type=float, help='Per cent probability of keeping any given read (pair) in [0,100]', metavar='FLOAT')
    options = parser.parse_args()

    random.seed(a=options.seed)
    seq_reader = sequences.file_reader(options.infile)
    fout = utils.open_file_write(options.outfile)

    if options.mate_file:
        mate_seq_reader = sequences.file_reader(options.mate_file)

    for seq in seq_reader:
        if options.mate_file:
            try:
                mate_seq = next(mate_seq_reader)
            except StopIteration:
                print('Error! Didn\'t get mate for read', seq.id, file=sys.stderr)
                sys.exit(1)
        if 100 * random.random() <= options.percent:
            print(seq, file=fout)
            if options.mate_file:
                print(mate_seq, file=fout)

    utils.close(fout)

    def test_file_reader_gff(self):
        '''Test read gff file'''
        good_files = [
            'sequences_test_gffv3.gff',
            'sequences_test_gffv3.no_FASTA_line.gff'
        ]
        good_files = [os.path.join(data_dir, x) for x in good_files]

        for f in good_files:
            reader = sequences.file_reader(f)
            counter = 1
            for seq in reader:
                self.assertEqual(seq, sequences.Fasta('seq' + str(counter), 'ACGTACGTAC'))
                counter += 1

        bad_files = [
            'sequences_test_gffv3.no_seq.gff',
            'sequences_test_gffv3.no_seq.2.gff'
        ]
        bad_files = [os.path.join(data_dir, x) for x in bad_files]

        for filename in bad_files:
            with self.assertRaises(sequences.Error):
                reader = sequences.file_reader(filename)
                for seq in reader:
                    pass

def interleave(infile_1, infile_2, outfile):
    seq_reader_1 = sequences.file_reader(infile_1)
    seq_reader_2 = sequences.file_reader(infile_2)
    f_out = utils.open_file_write(outfile)

    for seq_1 in seq_reader_1:
        try:
            seq_2 = next(seq_reader_2)
        except:
            utils.close(f_out)
            raise Error('Error getting mate for sequence', seq_1.id, ' ... cannot continue')

        print(seq_1, file=f_out)
        print(seq_2, file=f_out)

    try:
        seq_2 = next(seq_reader_2)
    except:
        seq_2 = None

    if seq_2 is not None:
        utils.close(f_out)
        raise Error('Error getting mate for sequence', seq_2.id, ' ... cannot continue')

    utils.close(f_out)

def interleave(infile_1, infile_2, outfile, suffix1=None, suffix2=None):
    '''Makes interleaved file from two sequence files. If used, will append suffix1 onto end
    of every sequence name in infile_1, unless it already ends with suffix1. Similar for sufffix2.'''
    seq_reader_1 = sequences.file_reader(infile_1)
    seq_reader_2 = sequences.file_reader(infile_2)
    f_out = utils.open_file_write(outfile)

    for seq_1 in seq_reader_1:
        try:
            seq_2 = next(seq_reader_2)
        except:
            utils.close(f_out)
            raise Error('Error getting mate for sequence', seq_1.id, ' ... cannot continue')

        if suffix1 is not None and not seq_1.id.endswith(suffix1):
            seq_1.id += suffix1
        if suffix2 is not None and not seq_2.id.endswith(suffix2):
            seq_2.id += suffix2

        print(seq_1, file=f_out)
        print(seq_2, file=f_out)

    try:
        seq_2 = next(seq_reader_2)
    except:
        seq_2 = None

    if seq_2 is not None:
        utils.close(f_out)
        raise Error('Error getting mate for sequence', seq_2.id, ' ... cannot continue')

    utils.close(f_out)

def filter(
      infile,
      outfile,
      minlength=0,
      maxlength=float('inf'),
      regex=None,
      ids_file=None,
      invert=False,
      mate_in=None,
      mate_out=None,
      both_mates_pass=True,
    ):

    ids_from_file = set()
    if ids_file is not None:
        f = utils.open_file_read(ids_file)
        for line in f:
            ids_from_file.add(line.rstrip())
        utils.close(f)

    if mate_in:
        if mate_out is None:
            raise Error('Error in filter! mate_in provided. Must also provide mate_out')

        seq_reader_mate = sequences.file_reader(mate_in)
        f_out_mate = utils.open_file_write(mate_out)

    seq_reader = sequences.file_reader(infile)
    f_out = utils.open_file_write(outfile)
    if regex is not None:
        r = re.compile(regex)


    def passes(seq):
        return minlength <= len(seq) <= maxlength \
              and (regex is None or r.search(seq.id) is not None) \
              and (ids_file is None or seq.id in ids_from_file)

    for seq in seq_reader:
        seq_passes = passes(seq)
        if mate_in:
            try:
                seq_mate = next(seq_reader_mate)
            except:
                utils.close(f_out)
                raise Error('Error getting mate for sequence', seq.id, ' ... cannot continue')

            mate_passes = passes(seq_mate)
            want_the_pair = (seq_passes and mate_passes) \
                            or (( seq_passes or mate_passes) and not both_mates_pass)
            if want_the_pair != invert:
                print(seq, file=f_out)
                print(seq_mate, file=f_out_mate)
        elif seq_passes != invert:
            print(seq, file=f_out)
    utils.close(f_out)
    if mate_in:
        utils.close(f_out_mate)

def fasta_to_fastq(fasta_in, qual_in, outfile):
    fa_reader = sequences.file_reader(fasta_in)
    qual_reader = sequences.file_reader(qual_in, read_quals=True)
    f_out = utils.open_file_write(outfile)

    for seq in fa_reader:
        qual = next(qual_reader)
        if seq.id != qual.id:
            utils.close(f_out)
            raise Error('Mismatch in names from fasta and qual file', seq.id, qual.id)

        qual.seq = [int(x) for x in qual.seq.split()]
        print(seq.to_Fastq(qual.seq), file=f_out)

    utils.close(f_out)

def acgtn_only(infile, outfile):
    '''Replace every non-acgtn (case insensitve) character with an N'''
    f = utils.open_file_write(outfile)
    for seq in sequences.file_reader(infile):
        seq.replace_non_acgt()
        print(seq, file=f)
    utils.close(f)

def count_sequences(infile):
    '''Returns the number of sequences in a file'''
    seq_reader = sequences.file_reader(infile)
    n = 0
    for seq in seq_reader:
        n += 1
    return n

def trim_contigs(infile, outfile, trim):
    seq_reader = sequences.file_reader(infile)
    fout = utils.open_file_write(outfile)

    for seq in seq_reader:
        if len(seq) < 2 * trim:
            continue

        gaps = seq.gaps()
        bases = list(seq.seq)

        # extend the length of each gap
        for gap in gaps:
            left_start = max(gap.start - trim, 0)
            right_end = min(gap.end + trim + 1, len(seq))

            for i in range(left_start, gap.start):
                bases[i] = 'N'

            for i in range(gap.end, right_end):
                bases[i] = 'N'

        seq.seq = ''.join(bases)

        # trim start/end bases and tidy up any resulting Ns at either end of the trimmed seq
        seq.trim(trim, trim)
        seq.trim_Ns()

        # check that there is some non-N sequence left over
        regex = re.compile('[^nN]')
        if regex.search(seq.seq) is not None:
            print(seq, file=fout)

    utils.close(fout)

 def test_file_reader_fasta(self):
     '''file_reader should iterate through a fasta file correctly'''
     reader = sequences.file_reader(os.path.join(data_dir, 'sequences_test.fa'))
     counter = 1
     for seq in reader:
         self.assertEqual(seq, sequences.Fasta(str(counter), 'ACGTA'))
         counter += 1

def to_fasta(infile, outfile, line_length=60, strip_after_first_whitespace=False, check_unique=False):
    seq_reader = sequences.file_reader(infile)
    f_out = utils.open_file_write(outfile)
    original_line_length = sequences.Fasta.line_length
    sequences.Fasta.line_length = line_length
    if check_unique:
        used_names = {}

    for seq in seq_reader:
        if strip_after_first_whitespace:
            seq.strip_after_first_whitespace()

        if check_unique:
            used_names[seq.id] = used_names.get(seq.id, 0) + 1

        if type(seq) == sequences.Fastq:
            print(sequences.Fasta(seq.id, seq.seq), file=f_out)
        else:
            print(seq, file=f_out)

    utils.close(f_out)
    sequences.Fasta.line_length = original_line_length

    if check_unique:
        all_unique = True

        for name, count in used_names.items():
            if count > 1:
                print('Sequence name "' + name + '" not unique. Found', count, 'times', file=sys.stderr)
                all_unique = False

        if not all_unique:
            raise Error('Not all sequence names unique. Cannot continue')

def translate(infile, outfile, frame=0):
    seq_reader = sequences.file_reader(infile)
    fout = utils.open_file_write(outfile)

    for seq in seq_reader:
        print(seq.translate(frame=frame), file=fout)

    utils.close(fout)

def reverse_complement(infile, outfile):
    seq_reader = sequences.file_reader(infile)
    fout = utils.open_file_write(outfile)

    for seq in seq_reader:
        seq.revcomp()
        print(seq, file=fout)

    utils.close(fout)

def replace_bases(infile, outfile, old, new):
    seq_reader = sequences.file_reader(infile)
    f_out = utils.open_file_write(outfile)

    for seq in seq_reader:
        seq.replace_bases(old, new)
        print(seq, file=f_out)

    utils.close(f_out)

def strip_illumina_suffix(infile, outfile):
    seq_reader = sequences.file_reader(infile)
    f_out = utils.open_file_write(outfile)

    for seq in seq_reader:
        seq.strip_illumina_suffix()
        print(seq, file=f_out)

    utils.close(f_out)