C++ p7_hmm_Destroy函数代码示例

static void
utest_SendRecv(ESL_RANDOMNESS *rng, int my_rank, int nproc)
{
  char            msg[] = "utest_SendRecv() failed";
  ESL_ALPHABET   *abc   = esl_alphabet_Create(eslAMINO);
  P7_HMM         *hmm   = NULL;
  P7_HMM         *xhmm  = NULL;
  int             M     = 200;
  char           *wbuf  = NULL;
  int             wn    = 0;
  int             i;
  uint32_t        rngseed;
  MPI_Status      mpistatus;
  char            errmsg[eslERRBUFSIZE];

  if (my_rank == 0) 
    {
      /* First we send our RNG seed to all workers */
      rngseed = esl_randomness_GetSeed(rng);
      for (i = 1; i < nproc; i++)
	if (MPI_Send( &rngseed, 1, MPI_UNSIGNED, i, 0, MPI_COMM_WORLD) != MPI_SUCCESS) esl_fatal(msg);

      /* We sample an HMM that's going to be identical to the workers' */
      if (p7_modelsample(rng, M, abc, &hmm) != eslOK) esl_fatal(msg);

      for (i = 1; i < nproc; i++)
	{
	  if (p7_hmm_mpi_Recv(MPI_ANY_SOURCE, 0, MPI_COMM_WORLD, &wbuf, &wn, &abc, &xhmm) != eslOK) esl_fatal(msg);

	  if (p7_hmm_Validate(xhmm, errmsg, 0.001) != eslOK) esl_fatal("%s:\n   %s", msg, errmsg);
	  if (p7_hmm_Compare(hmm, xhmm, 0.001)     != eslOK) esl_fatal(msg);

	  p7_hmm_Destroy(xhmm);
	}
    }
  else 
    {
      /* Worker(s) must first receive the exact same RNG seed that the master is using. */
      if (MPI_Recv(&rngseed, 1, MPI_UNSIGNED, 0, 0, MPI_COMM_WORLD, &mpistatus) != MPI_SUCCESS) esl_fatal(msg);

      /* and then the worker(s) can create the exact same RNG (and random number sequence) that the master has */
      rng = esl_randomness_CreateFast(rngseed);

      /* so when the worker samples this HMM, the master has independently sampled an exact duplicate of it... */
      if (p7_modelsample(rng, M, abc, &hmm) != eslOK) esl_fatal(msg);

      /* each worker sends the HMM to the master (it's the same HMM for each worker. The test is intended for one master, one worker.) */
      if (p7_hmm_mpi_Send(hmm, 0, 0, MPI_COMM_WORLD, &wbuf, &wn) != eslOK) esl_fatal(msg);

      /* worker's RNG is a private copy; destroy it. Master keeps its RNG, which the caller is responsible for. */
      esl_randomness_Destroy(rng);
     }

  p7_hmm_Destroy(hmm);
  esl_alphabet_Destroy(abc);
  free(wbuf);
  return;
}

/* mpi_worker()
 * The main control for an MPI worker process.
 */
static void
mpi_worker(ESL_GETOPTS *go, struct cfg_s *cfg)
{
  int             xstatus = eslOK;
  int             status;
  P7_HMM         *hmm     = NULL;
  char           *wbuf    = NULL;
  double         *xv      = NULL; /* result: array of N scores */
  int            *av      = NULL; /* optional result: array of N alignment lengths */
  int             wn      = 0;
  char            errbuf[eslERRBUFSIZE];
  int             pos;
 
  /* Worker initializes */
  if ((status = minimum_mpi_working_buffer(go, cfg->N, &wn)) != eslOK) xstatus = status;
  ESL_ALLOC(wbuf, wn * sizeof(char));
  ESL_ALLOC(xv,   cfg->N * sizeof(double) + 2);	
  if (esl_opt_GetBoolean(go, "-a"))
    ESL_ALLOC(av, cfg->N * sizeof(int));

  /* Main worker loop */
  while (p7_hmm_mpi_Recv(0, 0, MPI_COMM_WORLD, &wbuf, &wn, &(cfg->abc), &hmm) == eslOK) 
    {
      if (esl_opt_GetBoolean(go, "--recal")) {
	if (( status = recalibrate_model(go, cfg, errbuf, hmm))     != eslOK) goto CLEANERROR;
      }
      if ((status = process_workunit(go, cfg, errbuf, hmm, xv, av)) != eslOK) goto CLEANERROR;

      pos = 0;
      MPI_Pack(&status, 1,      MPI_INT,    wbuf, wn, &pos, MPI_COMM_WORLD);
      MPI_Pack(xv,      cfg->N, MPI_DOUBLE, wbuf, wn, &pos, MPI_COMM_WORLD);
      if (esl_opt_GetBoolean(go, "-a"))
	MPI_Pack(av,    cfg->N, MPI_INT,    wbuf, wn, &pos, MPI_COMM_WORLD);
      MPI_Send(wbuf, pos, MPI_PACKED, 0, 0, MPI_COMM_WORLD);
      p7_hmm_Destroy(hmm);
    }

  free(wbuf);
  free(xv);
  if (av != NULL) free(av);
  return;

 CLEANERROR:
  pos = 0;
  MPI_Pack(&status, 1,                MPI_INT,  wbuf, wn, &pos, MPI_COMM_WORLD);
  MPI_Pack(errbuf,  eslERRBUFSIZE,    MPI_CHAR, wbuf, wn, &pos, MPI_COMM_WORLD);
  MPI_Send(wbuf, pos, MPI_PACKED, 0, 0, MPI_COMM_WORLD);
  if (wbuf != NULL) free(wbuf);
  if (hmm  != NULL) p7_hmm_Destroy(hmm);
  if (xv   != NULL) free(xv);
  if (av   != NULL) free(av);
  return;

 ERROR:
  p7_Fail("Allocation error in mpi_worker");
}

/* Function:  p7_Builder()
 * Synopsis:  Build a new HMM from an MSA.
 *
 * Purpose:   Take the multiple sequence alignment <msa> and a build configuration <bld>,
 *            and build a new HMM. 
 * 
 *            Effective sequence number determination and calibration steps require
 *            additionally providing a null model <bg>.
 *
 * Args:      bld         - build configuration
 *            msa         - multiple sequence alignment
 *            bg          - null model
 *            opt_hmm     - optRETURN: new HMM
 *            opt_trarr   - optRETURN: array of faux tracebacks, <0..nseq-1>
 *            opt_gm      - optRETURN: profile corresponding to <hmm>
 *            opt_om      - optRETURN: optimized profile corresponding to <gm>
 *            opt_postmsa - optRETURN: RF-annotated, possibly modified MSA 
 *
 * Returns:   <eslOK> on success. The new HMM is optionally returned in
 *            <*opt_hmm>, along with optional returns of an array of faux tracebacks
 *            for each sequence in <*opt_trarr>, the annotated MSA used to construct
 *            the model in <*opt_postmsa>, a configured search profile in 
 *            <*opt_gm>, and an optimized search profile in <*opt_om>. These are
 *            all optional returns because the caller may, for example, be interested
 *            only in an optimized profile, or may only be interested in the HMM.
 *            
 *            Returns <eslENORESULT> if no consensus columns were annotated.
 *            Returns <eslEFORMAT> on MSA format problems, such as a missing RF annotation
 *            line in hand architecture construction. On any returned error,
 *            <bld->errbuf> contains an informative error message.
 *
 * Throws:    <eslEMEM> on allocation error.
 *            <eslEINVAL> if relative weights couldn't be calculated from <msa>.
 *
 * Xref:      J4/30.
 */
int
p7_Builder(P7_BUILDER *bld, ESL_MSA *msa, P7_BG *bg,
	   P7_HMM **opt_hmm, P7_TRACE ***opt_trarr, P7_PROFILE **opt_gm, P7_OPROFILE **opt_om,
	   ESL_MSA **opt_postmsa)
{
  int i,j;
  uint32_t    checksum = 0;	/* checksum calculated for the input MSA. hmmalign --mapali verifies against this. */
  P7_HMM     *hmm      = NULL;
  P7_TRACE  **tr       = NULL;
  P7_TRACE ***tr_ptr   = (opt_trarr != NULL || opt_postmsa != NULL) ? &tr : NULL;
  int         status;
  if ((status =  validate_msa         (bld, msa))                       != eslOK) goto ERROR;
  if ((status =  esl_msa_Checksum     (msa, &checksum))                 != eslOK) ESL_XFAIL(status, bld->errbuf, "Failed to calculate checksum"); 
  if ((status =  relative_weights     (bld, msa))                       != eslOK) goto ERROR;
  if ((status =  esl_msa_MarkFragments(msa, bld->fragthresh))           != eslOK) goto ERROR;
  if ((status =  build_model          (bld, msa, &hmm, tr_ptr))         != eslOK) goto ERROR;

  //Ensures that the weighted-average I->I count <=  bld->max_insert_len
  //(MI currently contains the number of observed insert-starts)
  if (bld->max_insert_len>0)
    for (i=1; i<hmm->M; i++ )
      hmm->t[i][p7H_II] = ESL_MIN(hmm->t[i][p7H_II], bld->max_insert_len*hmm->t[i][p7H_MI]);

  if ((status =  effective_seqnumber  (bld, msa, hmm, bg))              != eslOK) goto ERROR;
  if ((status =  parameterize         (bld, hmm))                       != eslOK) goto ERROR;
  if ((status =  annotate             (bld, msa, hmm))                  != eslOK) goto ERROR;
  if ((status =  calibrate            (bld, hmm, bg, opt_gm, opt_om))   != eslOK) goto ERROR;
  if ((status =  make_post_msa        (bld, msa, hmm, tr, opt_postmsa)) != eslOK) goto ERROR;

  //force masked positions to background  (it'll be close already, so no relevant impact on weighting)
  if (hmm->mm != NULL)
    for (i=1; i<hmm->M; i++ )
      if (hmm->mm[i] == 'm')
        for (j=0; j<hmm->abc->K; j++)
          hmm->mat[i][j] = bg->f[j];

  if ( bld->abc->type == eslDNA ||  bld->abc->type == eslRNA ) {
	  if (bld->w_len > 0)           hmm->max_length = bld->w_len;
	  else if (bld->w_beta == 0.0)  hmm->max_length = hmm->M *4;
	  else if ( (status =  p7_Builder_MaxLength(hmm, bld->w_beta)) != eslOK) goto ERROR;
  }

  hmm->checksum = checksum;
  hmm->flags   |= p7H_CHKSUM;

  if (opt_hmm   != NULL) *opt_hmm   = hmm; else p7_hmm_Destroy(hmm);
  if (opt_trarr != NULL) *opt_trarr = tr;  else p7_trace_DestroyArray(tr, msa->nseq);
  return eslOK;

 ERROR:
  p7_hmm_Destroy(hmm);
  p7_trace_DestroyArray(tr, msa->nseq);
  if (opt_gm    != NULL) p7_profile_Destroy(*opt_gm);
  if (opt_om    != NULL) p7_oprofile_Destroy(*opt_om);
  return status;
}

/* Function:  p7_SingleBuilder()
 * Synopsis:  Build a new HMM from a single sequence.
 *
 * Purpose:   Take the sequence <sq> and a build configuration <bld>, and
 *            build a new HMM.
 *            
 *            The single sequence scoring system in the <bld>
 *            configuration must have been previously initialized by
 *            <p7_builder_SetScoreSystem()>.
 *            
 * Args:      bld       - build configuration
 *            sq        - query sequence
 *            bg        - null model (needed to paramaterize insert emission probs)
 *            opt_hmm   - optRETURN: new HMM
 *            opt_gm    - optRETURN: profile corresponding to <hmm>
 *            opt_om    - optRETURN: optimized profile corresponding to <gm>
 *
 * Returns:   <eslOK> on success.
 *
 * Throws:    <eslEMEM> on allocation error.
 *            <eslEINVAL> if <bld> isn't properly configured somehow.
 */
int
p7_SingleBuilder(P7_BUILDER *bld, ESL_SQ *sq, P7_BG *bg, P7_HMM **opt_hmm,
		 P7_TRACE **opt_tr, P7_PROFILE **opt_gm, P7_OPROFILE **opt_om)
{
  P7_HMM   *hmm = NULL;
  P7_TRACE *tr  = NULL;
  int       k;
  int       status;
  
  bld->errbuf[0] = '\0';
  if (! bld->Q) ESL_XEXCEPTION(eslEINVAL, "score system not initialized");

  if ((status = p7_Seqmodel(bld->abc, sq->dsq, sq->n, sq->name, bld->Q, bg->f, bld->popen, bld->pextend, &hmm)) != eslOK) goto ERROR;
  if ((status = p7_hmm_SetComposition(hmm))                                                                     != eslOK) goto ERROR;
  if ((status = p7_hmm_SetConsensus(hmm, sq))                                                                   != eslOK) goto ERROR; 
  if ((status = calibrate(bld, hmm, bg, opt_gm, opt_om))                                                        != eslOK) goto ERROR;

  if ( bld->abc->type == eslDNA ||  bld->abc->type == eslRNA ) {
    if (bld->w_len > 0)           hmm->max_length = bld->w_len;
    else if (bld->w_beta == 0.0)  hmm->max_length = hmm->M *4;
    else if ( (status =  p7_Builder_MaxLength(hmm, bld->w_beta)) != eslOK) goto ERROR;
  }


  /* build a faux trace: relative to core model (B->M_1..M_L->E) */
  if (opt_tr != NULL) 
    {
      if ((tr = p7_trace_Create())                      == NULL)  goto ERROR;
      if ((status = p7_trace_Append(tr, p7T_B, 0, 0))   != eslOK) goto ERROR; 
      for (k = 1; k <= sq->n; k++)
        if ((status = p7_trace_Append(tr, p7T_M, k, k)) != eslOK) goto ERROR;
      if ((status = p7_trace_Append(tr, p7T_E, 0, 0))   != eslOK) goto ERROR; 
      tr->M = sq->n;
      tr->L = sq->n;
    }

  /* note that <opt_gm> and <opt_om> were already set by calibrate() call above. */
  if (opt_hmm   != NULL) *opt_hmm = hmm; else p7_hmm_Destroy(hmm);
  if (opt_tr    != NULL) *opt_tr  = tr;
  return eslOK;

 ERROR:
  p7_hmm_Destroy(hmm);
  if (tr        != NULL) p7_trace_Destroy(tr);
  if (opt_gm    != NULL) p7_profile_Destroy(*opt_gm);
  if (opt_om    != NULL) p7_oprofile_Destroy(*opt_om);
  return status;
}

/* utest_basic()
 * An MSA to ex{e,o}rcise past demons.
 *   1. seq2 gives an I->end transition.
 *   2. seq1 contains degenerate Z,X, exercising symbol counting
 *      of degenerate residues.
 */
static void
utest_basic(void)
{
  char         *failmsg      = "failure in build.c::utest_basic() unit test";
  char          msafile[16]  = "p7tmpXXXXXX"; /* tmpfile name template */
  FILE         *ofp          = NULL;
  ESL_ALPHABET *abc          = esl_alphabet_Create(eslAMINO);
  ESL_MSAFILE  *afp          = NULL;
  ESL_MSA      *msa          = NULL;
  P7_HMM       *hmm          = NULL;
  float         symfrac      = 0.5;

  if (esl_tmpfile_named(msafile, &ofp) != eslOK) esl_fatal(failmsg);
  fprintf(ofp, "# STOCKHOLM 1.0\n");
  fprintf(ofp, "#=GC RF --xxxxxxxxxxxxxxxx-xxx-x--\n");
  fprintf(ofp, "seq1    --ACDEFGHIKLMNPZXS-TVW-Yyy\n");
  fprintf(ofp, "seq2    aaACDEFGHIKLMNPQRS-TVWw---\n");
  fprintf(ofp, "seq3    aaAC-EFGHIKLMNPQRS-TVW-Y--\n");
  fprintf(ofp, "seq4    aaAC-EFGHIKLMNPQRS-TVW-Y--\n");
  fprintf(ofp, "//\n");
  fclose(ofp);

  if (esl_msafile_Open(&abc, msafile, NULL, eslMSAFILE_UNKNOWN, NULL, &afp) != eslOK) esl_fatal(failmsg);
  if (esl_msafile_Read(afp, &msa)                                           != eslOK) esl_fatal(failmsg);
  if (p7_Fastmodelmaker(msa, symfrac, NULL, &hmm, NULL)                     != eslOK) esl_fatal(failmsg);
  
  p7_hmm_Destroy(hmm);
  esl_msa_Destroy(msa);
  esl_msafile_Close(afp);
  esl_alphabet_Destroy(abc);
  remove(msafile);
  return;
}

static void 
utest_normalization(ESL_GETOPTS *go)
{
  char         *msg     = "seqmodel normalization utest failed";
  ESL_ALPHABET *abc     = esl_alphabet_Create(eslAMINO);
  char         *seq     = "ACDEFGHIKLMNPQRSTVWYBJZOUX";
  int           L       = strlen(seq);
  ESL_DSQ      *dsq     = NULL;
  float         popen   = 0.1;
  float         pextend = 0.4;
  P7_BUILDER   *bld     = NULL;
  P7_BG        *bg      = p7_bg_Create(abc);
  P7_HMM       *hmm     = NULL;
  char          errbuf[eslERRBUFSIZE];

  if ( esl_abc_CreateDsq(abc, seq, &dsq)                                                 != eslOK) esl_fatal(msg);
  if ( (bld = p7_builder_Create(NULL, abc))                                              == NULL)  esl_fatal(msg);
  if ( p7_builder_LoadScoreSystem(bld, "BLOSUM62", popen, pextend, bg)                   != eslOK) esl_fatal(msg); 
  if ( p7_Seqmodel(abc, dsq, L, "aatest", bld->Q, bg->f, bld->popen, bld->pextend, &hmm) != eslOK) esl_fatal(msg);

  if (p7_hmm_Validate(hmm, errbuf, 0.0001) != eslOK) esl_fatal("normalization utest failed\n%s\n", errbuf);

  free(dsq);
  p7_bg_Destroy(bg);
  p7_hmm_Destroy(hmm);
  p7_builder_Destroy(bld);
  esl_alphabet_Destroy(abc);
}

int 
main(int argc, char **argv)
{
  char           *msg  = "p7_gmx unit test driver failed";
  ESL_GETOPTS    *go   = p7_CreateDefaultApp(options, 0, argc, argv, banner, usage);
  ESL_RANDOMNESS *r    = esl_randomness_CreateFast(esl_opt_GetInteger(go, "-s"));
  ESL_ALPHABET   *abc  = esl_alphabet_Create(eslAMINO);
  P7_BG          *bg   = p7_bg_Create(abc);
  P7_HMM         *hmm  = NULL;
  P7_PROFILE     *gm   = NULL;
  int             M    = esl_opt_GetInteger(go, "-M");
  int             L    = esl_opt_GetInteger(go, "-L");
  float           tol  = esl_opt_GetReal   (go, "-t");

  p7_FLogsumInit();

  if (p7_hmm_Sample(r, M, abc, &hmm)                != eslOK) esl_fatal(msg);
  if ((gm = p7_profile_Create(hmm->M, abc))         == NULL)  esl_fatal(msg);
  if (p7_bg_SetLength(bg, L)                        != eslOK) esl_fatal(msg);
  if (p7_ProfileConfig(hmm, bg, gm, L, p7_UNILOCAL) != eslOK) esl_fatal(msg);

  utest_GrowTo();
  utest_Compare(r, gm, bg, L, tol);

  esl_getopts_Destroy(go);
  esl_randomness_Destroy(r);
  esl_alphabet_Destroy(abc);
  p7_bg_Destroy(bg);
  p7_hmm_Destroy(hmm);
  p7_profile_Destroy(gm);
  return eslOK;
}

int
main(int argc, char **argv)
{
  ESL_GETOPTS    *go   = esl_getopts_CreateDefaultApp(options, 0, argc, argv, banner, usage);
  ESL_RANDOMNESS *r    = esl_randomness_CreateFast(esl_opt_GetInteger(go, "-s"));
  ESL_ALPHABET   *abc  = NULL;
  P7_BG          *bg   = NULL;
  P7_HMM         *hmm  = NULL;
  P7_OPROFILE    *om   = NULL;
  int             M    = esl_opt_GetInteger(go, "-M");
  int             L    = esl_opt_GetInteger(go, "-L");
  
  /* Sample a random HMM and optimized profile, in amino acid alphabet.  */
  if ((abc = esl_alphabet_Create(eslAMINO))                    == NULL)  esl_fatal("failed to create alphabet");
  if ((bg = p7_bg_Create(abc))                                 == NULL)  esl_fatal("failed to create null model");
  if (( p7_oprofile_Sample(r, abc, bg, M, L, &hmm, NULL, &om)) != eslOK) esl_fatal("failed to sample HMM and profile");

  /* unit test(s) */
  utest_ReadWrite(hmm, om);

  p7_oprofile_Destroy(om);
  p7_hmm_Destroy(hmm);
  p7_bg_Destroy(bg);
  esl_alphabet_Destroy(abc);
  esl_randomness_Destroy(r);
  esl_getopts_Destroy(go);
  return eslOK;
}

static int
serial_loop(WORKER_INFO *info, struct cfg_s *cfg)
{
  P7_BUILDER *bld         = NULL;
  ESL_MSA    *msa         = NULL;
  ESL_MSA    *postmsa     = NULL;
  ESL_MSA   **postmsa_ptr = (cfg->postmsafile != NULL) ? &postmsa : NULL;
  P7_HMM     *hmm         = NULL;
  char        errmsg[eslERRBUFSIZE];
  int         status;

  double      entropy;

  cfg->nali = 0;
  while ((status = esl_msa_Read(cfg->afp, &msa)) == eslOK)
    {
      cfg->nali++;  

      if ((status = set_msa_name(cfg, errmsg, msa)) != eslOK) p7_Fail("%s\n", errmsg); /* cfg->nnamed gets incremented in this call */

                /*         bg   new-HMM trarr gm   om  */
      if ((status = p7_Builder(info->bld, msa, info->bg, &hmm, NULL, NULL, NULL, postmsa_ptr)) != eslOK) p7_Fail("build failed: %s", bld->errbuf);

      entropy = p7_MeanMatchRelativeEntropy(hmm, info->bg);
      if ((status = output_result(cfg, errmsg, cfg->nali, msa, hmm, postmsa, entropy))         != eslOK) p7_Fail(errmsg);

      p7_hmm_Destroy(hmm);
      esl_msa_Destroy(msa);
      esl_msa_Destroy(postmsa);
    }

  return status;
}

int
main(int argc, char **argv)
{
    ESL_GETOPTS    *go      = p7_CreateDefaultApp(options, 1, argc, argv, banner, usage);
    char           *hmmfile = esl_opt_GetArg(go, 1);
    ESL_STOPWATCH  *w       = esl_stopwatch_Create();
    ESL_RANDOMNESS *r       = esl_randomness_CreateFast(esl_opt_GetInteger(go, "-s"));
    ESL_ALPHABET   *abc     = NULL;
    P7_HMMFILE     *hfp     = NULL;
    P7_HMM         *hmm     = NULL;
    P7_BG          *bg      = NULL;
    P7_PROFILE     *gm      = NULL;
    P7_GMX         *gx1     = NULL;
    P7_GMX         *gx2     = NULL;
    int             L       = esl_opt_GetInteger(go, "-L");
    int             N       = esl_opt_GetInteger(go, "-N");
    ESL_DSQ        *dsq     = malloc(sizeof(ESL_DSQ) * (L+2));
    float           null2[p7_MAXCODE];
    int             i;
    float           fsc, bsc;
    double          Mcs;

    if (p7_hmmfile_OpenE(hmmfile, NULL, &hfp, NULL) != eslOK) p7_Fail("Failed to open HMM file %s", hmmfile);
    if (p7_hmmfile_Read(hfp, &abc, &hmm)            != eslOK) p7_Fail("Failed to read HMM");

    bg = p7_bg_Create(abc);
    p7_bg_SetLength(bg, L);
    gm = p7_profile_Create(hmm->M, abc);
    p7_ProfileConfig(hmm, bg, gm, L, p7_LOCAL);
    gx1 = p7_gmx_Create(gm->M, L);
    gx2 = p7_gmx_Create(gm->M, L);

    esl_rsq_xfIID(r, bg->f, abc->K, L, dsq);
    p7_GForward (dsq, L, gm, gx1, &fsc);
    p7_GBackward(dsq, L, gm, gx2, &bsc);
    p7_GDecoding(gm, gx1, gx2, gx2);

    esl_stopwatch_Start(w);
    for (i = 0; i < N; i++)
        p7_GNull2_ByExpectation(gm, gx2, null2);
    esl_stopwatch_Stop(w);

    Mcs  = (double) N * (double) L * (double) gm->M * 1e-6 / w->user;
    esl_stopwatch_Display(stdout, w, "# CPU time: ");
    printf("# M    = %d\n", gm->M);
    printf("# %.1f Mc/s\n", Mcs);

    free(dsq);
    p7_gmx_Destroy(gx1);
    p7_gmx_Destroy(gx2);
    p7_profile_Destroy(gm);
    p7_bg_Destroy(bg);
    p7_hmm_Destroy(hmm);
    p7_hmmfile_Close(hfp);
    esl_alphabet_Destroy(abc);
    esl_stopwatch_Destroy(w);
    esl_randomness_Destroy(r);
    esl_getopts_Destroy(go);
    return 0;
}

int 
main(int argc, char **argv)
{
  ESL_GETOPTS    *go      = p7_CreateDefaultApp(options, 1, argc, argv, banner, usage);
  char           *hmmfile = esl_opt_GetArg(go, 1);
  int             N       = esl_opt_GetInteger(go, "-N");
  ESL_STOPWATCH  *w       = esl_stopwatch_Create();
  ESL_ALPHABET   *abc     = NULL;
  P7_HMMFILE     *hfp     = NULL;
  P7_HMM         *hmm     = NULL;

  if (p7_hmmfile_OpenE(hmmfile, NULL, &hfp, NULL) != eslOK) p7_Fail("Failed to open HMM file %s", hmmfile);
  if (p7_hmmfile_Read(hfp, &abc, &hmm)            != eslOK) p7_Fail("Failed to read HMM");
  p7_hmmfile_Close(hfp);

  esl_stopwatch_Start(w);
  while (N--)
    { /*                cfg   rng   bg    gm    om  */
      p7_Calibrate(hmm, NULL, NULL, NULL, NULL, NULL);
    }
  esl_stopwatch_Stop(w);
  esl_stopwatch_Display(stdout, w, "# CPU time: ");

  p7_hmm_Destroy(hmm);
  esl_alphabet_Destroy(abc);
  esl_stopwatch_Destroy(w);
  esl_getopts_Destroy(go);
  return 0;
}

int
main(int argc, char **argv)
{
  ESL_GETOPTS    *go      = esl_getopts_CreateDefaultApp(options, 1, argc, argv, banner, usage);
  ESL_STOPWATCH  *w       = esl_stopwatch_Create();
  char           *hmmfile = esl_opt_GetArg(go, 1);
  ESL_ALPHABET   *abc     = NULL;
  P7_HMMFILE     *hfp     = NULL;
  P7_HMM         *hmm     = NULL;
  P7_BG          *bg      = NULL;
  int             L       = esl_opt_GetInteger(go, "-L");
  int             N       = esl_opt_GetInteger(go, "-N");
  int             i;
 
  /* Read one HMM from <hmmfile> */
  if (p7_hmmfile_Open(hmmfile, NULL, &hfp) != eslOK) p7_Fail("Failed to open HMM file %s", hmmfile);
  if (p7_hmmfile_Read(hfp, &abc, &hmm)     != eslOK) p7_Fail("Failed to read HMM");
  p7_hmmfile_Close(hfp);

  bg = p7_bg_Create(abc);

  esl_stopwatch_Start(w);
  for (i = 0; i < N; i++)
    p7_bg_SetFilterByHMM(bg, hmm);
  esl_stopwatch_Stop(w);
  esl_stopwatch_Display(stdout, w, "# CPU time: ");

  p7_bg_Destroy(bg);
  p7_hmm_Destroy(hmm);
  esl_alphabet_Destroy(abc);
  esl_stopwatch_Destroy(w);
  esl_getopts_Destroy(go);
  return 0;
}

/* Function:  p7_oprofile_Sample()
 * Synopsis:  Sample a random profile.
 * Incept:    MSF Tue Nov 3, 2009 [Janelia]
 *
 * Purpose:   Sample a random profile of <M> nodes for alphabet <abc>,
 *            using <r> as the source of random numbers. Parameterize
 *            it for generation of target sequences of mean length
 *            <L>. Calculate its log-odds scores using background
 *            model <bg>.
 *            
 * Args:      r       - random number generator
 *            abc     - emission alphabet 
 *            bg      - background frequency model
 *            M       - size of sampled profile, in nodes
 *            L       - configured target seq mean length
 *            opt_hmm - optRETURN: sampled HMM
 *            opt_gm  - optRETURN: sampled normal profile
 *            opt_om  - RETURN: optimized profile
 *
 * Returns:   <eslOK> on success.
 *
 * Throws:    (no abnormal error conditions)
 */
int
p7_oprofile_Sample(ESL_RANDOMNESS *r, const ESL_ALPHABET *abc, const P7_BG *bg, int M, int L,
		   P7_HMM **opt_hmm, P7_PROFILE **opt_gm, P7_OPROFILE **ret_om)
{
  P7_HMM         *hmm  = NULL;
  P7_PROFILE     *gm   = NULL;
  P7_OPROFILE    *om   = NULL;
  int             status;

  if ((gm = p7_profile_Create (M, abc)) == NULL)  { status = eslEMEM; goto ERROR; }
  if ((om = p7_oprofile_Create(M, abc)) == NULL)  { status = eslEMEM; goto ERROR; }

  if ((status = p7_hmm_Sample(r, M, abc, &hmm))             != eslOK) goto ERROR;
  if ((status = p7_ProfileConfig(hmm, bg, gm, L, p7_LOCAL)) != eslOK) goto ERROR;
  if ((status = p7_oprofile_Convert(gm, om))                != eslOK) goto ERROR;
  if ((status = p7_oprofile_ReconfigLength(om, L))          != eslOK) goto ERROR;

  if (opt_hmm != NULL) *opt_hmm = hmm; else p7_hmm_Destroy(hmm);
  if (opt_gm  != NULL) *opt_gm  = gm;  else p7_profile_Destroy(gm);
  *ret_om = om;
  return eslOK;

 ERROR:
  if (opt_hmm != NULL) *opt_hmm = NULL;
  if (opt_gm  != NULL) *opt_gm  = NULL;
  *ret_om = NULL;
  return status;
}

int
main(int argc, char **argv)
{
  ESL_GETOPTS    *go    = esl_getopts_CreateDefaultApp(options, 0, argc, argv, banner, usage);
  ESL_RANDOMNESS *r    = esl_randomness_CreateFast(esl_opt_GetInteger(go, "-s"));
  ESL_ALPHABET   *abc  = NULL;
  P7_HMM         *hmm  = NULL;
  P7_PROFILE     *gm   = NULL;
  P7_BG          *bg   = NULL;
  int             M    = 100;
  int             L    = 200;
  int             nseq = 20;
  char            errbuf[eslERRBUFSIZE];

  if ((abc = esl_alphabet_Create(eslAMINO))         == NULL)  esl_fatal("failed to create alphabet");
  if (p7_hmm_Sample(r, M, abc, &hmm)                != eslOK) esl_fatal("failed to sample an HMM");
  if ((bg = p7_bg_Create(abc))                      == NULL)  esl_fatal("failed to create null model");
  if ((gm = p7_profile_Create(hmm->M, abc))         == NULL)  esl_fatal("failed to create profile");
  if (p7_ProfileConfig(hmm, bg, gm, L, p7_LOCAL)    != eslOK) esl_fatal("failed to config profile");
  if (p7_hmm_Validate    (hmm, errbuf, 0.0001)      != eslOK) esl_fatal("whoops, HMM is bad!: %s", errbuf);
  if (p7_profile_Validate(gm,  errbuf, 0.0001)      != eslOK) esl_fatal("whoops, profile is bad!: %s", errbuf);

  utest_basic  (go);
  utest_viterbi(go, r, abc, bg, gm, nseq, L);

  p7_profile_Destroy(gm);
  p7_bg_Destroy(bg);
  p7_hmm_Destroy(hmm);
  esl_alphabet_Destroy(abc);
  esl_randomness_Destroy(r);
  esl_getopts_Destroy(go);
  return 0;
}

static void
utest_Compare(void)
{
  ESL_RANDOMNESS *r    = esl_randomness_CreateFast(42);
  ESL_ALPHABET   *abc  = esl_alphabet_Create(eslAMINO);
  P7_HMM         *hmm  = NULL;
  P7_BG          *bg   = NULL;
  P7_PROFILE     *gm   = NULL;
  P7_PROFILE     *gm2  = NULL;
  int             M    = 200;
  int             L    = 400;

  p7_modelsample(r, M, abc, &hmm); /* master and worker's sampled profiles are identical */
  bg  = p7_bg_Create(abc);

  gm  = p7_profile_Create(hmm->M, abc);
  gm2 = p7_profile_Create(hmm->M, abc);

  p7_profile_Config(gm,  hmm, bg);
  p7_profile_Config(gm2, hmm, bg);

  p7_profile_SetLength(gm,  L);
  p7_profile_SetLength(gm2, L);

  if (p7_profile_Compare(gm, gm2, 0.001) != eslOK) p7_Die("identical profile comparison failed");
  
  p7_profile_Destroy(gm);
  p7_profile_Destroy(gm2);
  p7_bg_Destroy(bg);
  p7_hmm_Destroy(hmm);
  esl_alphabet_Destroy(abc);
  esl_randomness_Destroy(r);
  return;
}